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SpectraMax® i3x Multi-Mode Microplate Reader

SpectraMax® i3x Multi-Mode Microplate Reader alat yang dapat mengukur absorbansi, fluoresensi, dan luminesensi dengan peningkatan yang tersedia termasuk western blot, pencitraan sel, dan kinetika cepat dengan injektor ditambah mode deteksi tambahan. 

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Spesifikasi SpectraMax® i3x Multi-Mode Microplate Reader

Read modes

  • Absorbance
  • Fluorescence (top/bottom read)
  • Luminescence (top read)
  • Time-Resolved Fluorescence (top read)
  • Fluorescence Polarization (top read)
  • HTRF (top read)
  • AlphaScreen (top read)

Wavelength ranges

  • Abs: 230 - 1000 nm
  • FL Ex: 250 - 830 nm
  • FL Em: 270 - 850 nm
  • Lumi: 300 - 850 nm

Wavelength selection

  • Cartridge filters and monochromator tunable in 1 nm increments

Absorbance photometric accuracy/linearity

  • < ±0.010 OD ±1.0%, 0 to 2 OD

Absorbance photometric precision/repeatability

  • < ±0.003 OD ±1.0%, 0 to 2 OD

Sensitivity top read optimized

  • FL: Mono: 0.5 pM fluorescein 96 well, 1 pM fluorescein 384 well; Cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well
  • Lumi: Mono: 3 pM ATP-Glow 96 well, 6 pM ATP-Glow 384 well; Cartridge: 20 amol ATP-flash
  • TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well
  • FP cartridge: 3 mP (75 μl) fluorescein 384 well, 6 mP (8μl) fluorescein 1536 well
  • AlphaScreen cartridge: <100 amol/well 384 well

Light source(s)

  • High power xenon flash lamp
  • LEDs in visible range

Detector(s)

  • Silicon photodiode
  • Cooled photomultiplier tube

Read times

  • Abs: 29 seconds
  • FL: 22 seconds
  • Lumi: 21 seconds
  • TRF: 21 seconds
  • FP: 38 seconds
  • AS: 22 seconds

Plate shaking

  • Linear
  • Orbital

Plate type(s)

  • 6-1536 well plates

Temperature control

  • Ambient + 4°C to 45°C

Dimensions

  • H 32.5 cm x W 39.2 cm x D 60.55 cm

Validation tools

  • Absorbance validation plate
  • Fluorescence validation plate
  • Luminescence validation plate
  • Multi-mode validation plate
  • SoftMax® Pro GxP Software

 

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