NGSBIO Library Quant Kit Blue for Illumina® Separate-ROX (500 x 20μL Reactions)
The NGSBIO Library Quant Kit contains all the components required for accurate and sensitive quantification of libraries prepared for Illumina® NGS systems. The kit uses qPCR to specifically quantify adapterligated DNA molecules, ensuring optimal cluster densities for improved sequencing efficiency and quality of data.
ReadmoreProduct data and images for NGSBIO Library Quant Kit Blue for Illumina® Separate-ROX (500 x 20μL Reactions)
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NCSBIO Library Quant Kit Blue for Illumina is available in Lo, Hi and Separate-ROX formats.
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Amplification curves and standard curve : An adapter-ligated library sample (purple) is run alongside six standard templates (grey) provided in the NGSBIO Library Quant Kit (a). The Cts of the amplification curve are plotted against the log of the concentration of the standard tempaltes. A linear curve is fitted through thr standards (b). The concentration of the unknown sample is the calculated from its position on the curve.
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High consistency and reproducibility of quantification : 16 adapter-ligated libraries are quantified using NGSBIO Library Quant Kit (purple). NEBNext Library Quant Kit (orange) and KAPA Library Quantification Kit (green). The NGSBIO Library Quant Kit shows less spread and greater consistency among replicates
Specification for NGSBIO Library Quant Kit Blue for Illumina® Separate-ROX (500 x 20μL Reactions)
Component
2x qPCRBIO SyGreen Blue Mix No-ROX
50μM ROX Additive
DNA Standards 1-5
10x Illumina® Primers
10x Dilution Buffer
100 Reactions 1 x 1mL
1 x 200µL
30 µL each
1 x 0.2Ml
1 x 0.6mL
500 Reactions 5 x 1mL
1 x 200µL
85 µL each
1 x 1mL
2 x 1.5mL