MACSprep™ PBMC Isolation Kit, human
The MACSprep™ PBMC Isolation Kit, for the isolation of untouched PBMCs from whole blood. Non-PBMCs cells, such as neutrophils, eosinophils, platelets, and erythrocytes are magnetically labeled by using a cocktail of antibodies against CD15, CD61, CD66b, and CD235a. Isolation of highly pure PBMCs is achieved by depletion of the magnetically labeled cells.
Readmore
Specification for MACSprep™ PBMC Isolation Kit, human
Background information
The isolation of human peripheral blood mononuclear cells (PBMCs) is performed with only one labeling step and in a two-step separation procedure. During the first isolation step erythrocytes are aggregated and sedimented. In a second step, PBMCs are isolated by depletion of non-PBMCs (e.g. neutrophils, eosinophils, platelets, and residual erythrocytes). Non-PBMCs are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies and MACSprep™ Anti-Biotin MicroBeads. The magnetically labeled non-PBMCs are depleted by retaining them within a MACS Column in the magnetic field of a MACS® Separator while the unlabeled PBMCs run through.
Downstream applications
The MACSprep™ PBMC Isolation Kit, human is used for the fast isolation of human peripheral blood mononuclear cells (PBMCs).
Applications include:
- Efficient, fast, and convenient isolation of untouched PBMCs from blood samples of 1–8 mL
- PBMC co-cultures, e.g., functional immune cell assays
- Studies on cytokine expression of immune cells upon restimulation
- Studies on signal transduction during activation of immune cells
- Immune monitoring of antigen-specific T cells