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Laemmli Buffer 10x, for SDS PAGE (2L)

To obtain optimal protein separation in vertical SDS PAGE it is crucial to use the appropriate buffer system. The standard Tris/Glycine (Laemmli) buffer system is suitable for the MW range 7 – 200 kDa. Supplied as 10 x concentrate. Contains 0.25 M Tris, 1.92 M glycine and 1 % SDS in aqueous solution.

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Specification for Laemmli Buffer 10x, for SDS PAGE (2L)

Volume

  • 2L

Composition

Components Concentration
TRIS 0.25 M
Glycin 1.92 M
SDS 1 %
pH 8.4 – 8.9

Storage Temperature

  • The recommended storage temperature is +15 °C – +30 °C.
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